Anthurium ‘IAC Eidibel’ micropropagation by etiolation induction and plantlets regeneration

The commercial micropropagation of Anthurium has been made by indirect organogenesis, which can generate somaclonal variations. Aiming to reduce this problem, the objective of this work was to develop a protocol for in vitro multiplication of A. andraeanum var. IAC Eidibel by etiolation and following plantlets regeneration. For etiolation, individual stem segments without leaves, with 3 to 5 buds, were inoculated in test tube with 10 mL of MS or Pierik media, without auxin or with IAA, IBA or NAA at 10 µM.  The cultures were kept for 60 days at 25 ± 1ºC, in the dark. For plantlet regeneration, etiolated shoots were divided in segments of two buds and inoculated individually, in horizontal position, in test tubes with the same media, with BAP at 0; 2.22 µM; 4.44 µM or 6.66 µM. The cultures were kept in growth room under 30 μmol.m-2s-1 of light intensity, photoperiod of 16 hours and temperature of 25 ± 1ºC, for 60 days. The ANOVA was made and the means were compared by Tukey´s test (p<0.05). For the etiolation, Pierik medium was better than the MS medium for the following parameters: shoot length, number of node/shoot, total number of node/explant and internode length. The media without auxin differed from the media with NAA at 10 µM for all analyzed parameters. For plantlets regeneration, the media without BAP resulted in lower multiplication rates. It is recommended Pierik medium without auxin for etiolation, and the same medium added with BAP at 2.22 mM, for plantlet regeneration.  

ISSN: 2447-536X

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